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PhosphoSolutions/GABAA Receptor γ2 Antibody, 100 µl/832-GG2C/100 µl
Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000IHC1:300
- Species:
- Rabbit
- GeneName:
- GABRG2
- MolecularReference:
- ~46kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#832-GG2C,RRID:AB_2492115
Antigen/Purification:Collapse
Theantigenisa Fusionproteinfromthecytoplasmicloopoftheγ2-subunitofratGABAA receptor.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAA receptor(GABAA-R)subtype.GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depression,andsubstanceabuse.TheGABAA-Risamultimericsubunitcomplex.Todatesixαs,fourβsandfourγs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004).InjectioninoocytesormammaliancelllinesofcRNAcodingforα-andβ-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,coexpressionofaγ-subunitisrequiredforbenzodiazepinemodulation.Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferentα-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
González,M.I.,Grabenstatter,H.L.,Cea-DelRio,C.A.,DelAngel,Y.C.,Carlsen,J.,Laoprasert,R.P.,WhiteA.M.,HuntsmanM.M.,&Brooks-Kayal,A.(2015).Seizure-relatedregulationofGABAAreceptorsinspontaneouslyepilepticrats.Neurobiologyofdisease,77,246-256.PMID:25769812
GomezAM,FroemkeRC,BurdenSJ.(2014)SynapticplasticityandcognitivefunctionaredisruptedintheabsenceofLrp4.Elife.2014Nov19;3.PMID:25407677
Mao,Y.T.,&Pallas,S.L.(2013).Cross-modalplasticityresultsinincreasedinhibitioninprimaryauditorycorticalareas.Neuralplasticity,2013.PMID:24288625
Wen-HaiChou,DanWang,ThomasMcMahon,Zhan-HengQi,MaengseokSong,ChaoZhang,KevanM.Shokat,andRobertO.Messing(2010)GABAAReceptorTraffickingIsRegulatedbyProteinKinaseCandtheN-Ethylmaleimide-SensitiveFactorJ.Neurosci.,30:13955–13965.PMID:20962217
PöltlA,HauerB,FuchsK,TretterV,SieghartW(2003)SubunitcompositionandquantitativeimportanceofGABAAreceptorsubtypesinthecerebellumofmouseandrat.JNeuRochem87:1444-1455.PMID:14713300
WhitingPJ,BonnertTP,McKernanRM,FarrarS,LeBourdellèsB,HeavensRP,SmithDW,HewsonL,RigbyMR,SirinathsinghjiDJS,ThompsonSA,WaffordKA(1999)MolecularandfunctionaldiversityoftheexpandingGABAAreceptorgenefamily.AnnNYAcadSci868:645-653.PMID:10414349
