RabbitPolyclonalAntibody
*Thisantibodyisareplacementforouroriginalcatalog#868-GDN.
Itwasproducedbythesamemethods,usingthesamefusionprotein
antigeninnewanimals.
- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Mouse,Rat
- Applications:
- WB1:1000
- Species:
- Rabbit
- GeneName:
- GABRD
- MolecularReference:
- ~50kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#868A-GDN,RRID:AB_2631037
Antigen/Purification:Collapse
TheantigenisafusionproteinfromtheN-terminalregionoftheδ-subunitofratGABAAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Gamma-aminobutyricacid(GABA)istheprimaryinhibitoryneurotransmitterinthecentralnervoussystem,causingahyperpolarizationofthemembranethroughtheopeningofaCl−channelassociatedwiththeGABAAreceptor(GABAA-R)subtype. GABAA-Rsareimportanttherapeutictargetsforarangeofsedative,anxiolytic,andhypnoticagentsandareimplicatedinseveraldiseasesincludingepilepsy,anxiety,depressionandsubstanceabuse. TheGABAA-Risamultimericsubunitcomplex.Todatesixas,fourbsandfourgs,plusalternativesplicingvariantsofsomeofthesesubunits,havebeenidentified(OlsenandTobin,1990;Whitingetal.,1999;Ogrisetal.,2004). InjectioninoocytesormammaliancelllinesofcRNAcodingfora-andb-subunitsresultsintheexpressionoffunctionalGABAA-RssensitivetoGABA.However,co-expressionofag-subunitisrequiredforbenzodiazepinemodulation. Thevariouseffectsofthebenzodiazepinesinbrainmayalsobemediatedviadifferenta-subunitsofthereceptor(McKernanetal.,2000;MehtaandTicku,1998;Ogrisetal.,2004;Pöltletal.,2003). Morerecentlytherehavebeenanumberofstudiesdemonstratingthattheδ-subunitofthereceptormayaffectsubunitassembly(Korpietal.,2002)andmayalsoconferdifferentialsensitivitytoneurosteroidsandtoethanol(Wallneretal.,2003;Wohlfarthetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
KorpiER,MihalekRM,SinkkonenST,HauerB,HeversW,HomanicsGE,SieghartW,LuddensH(2002)AlteredreceptorsubtypesintheforebrainofGABAAreceptorδ-subunit-deficientmice:recruitmentofγ2-subunits.Neurosci109:733-743.
McKernanRM,etal.(2000)SedativebutnotanxiolyticpropertiesofbenzodiazepinesaremediatedbytheGABAAreceptorα1-subtype.NatureNeurosci3:587-592.
MehtaAK,TickuMK(1998)PrevalenceoftheGABAAreceptorassembliescontainingα1-subunitintheratcerebellumandcerebralcortexasdeterminedbyimmunoprecipitation:LackofmodulationbychronicethanoladmiNISTration.MolBrainRes67:194-199.
OgrisW,PöltlA,HauerB,ErnstM,ObertoA,WulffP,HögerH,WisdenW,SieghartW(2004)AffinityofvariousbenzodiazepinesiteligandsinmicewithapointmutationintheGABAAreceptorγ2-subunit.BiochemPharmacol68:1621-1629.
OlsenRW,TobinAJ(1990)MolecularbiologyofGABAAreceptors.FASEB4:1469-1480.
PöltlA,HauerB,FuchsK,TretterV,SieghartW(2003)SubunitcompositionandquantitativeimportanceofGABAAreceptorsubtypesinthecerebellumofmouseandrat.JNeuRochem87:1444-1455.
WallnerM,HancharHJ,OlsenRW(2003)FromTheCover:Ethanolenhancesα4β3δandα6β3δγ-aminobutyricacidtypeAreceptorsatlowconcentrationsknowntoaffecthumans.ProcNatlAcadSci(USA)100:15218-15223.
WhitingPJ,BonnertTP,McKernanRM,FarrarS,LeBourdellèsB,HeavensRP,SmithDW,HewsonL,RigbyMR,SirinathsinghjiDJS,ThompsonSA,WaffordKA(1999)MolecularandfunctionaldiversityoftheexpandingGABAAreceptorgenefamily.AnnNYAcadSci868:645-653.
WohlfarthKM,BianchiMT,MacdonaldRL(2002)EnhancedNeurosteroidPotentiationofTernaryGABAAReceptorsContainingtheδ-Subunit.JNeurosci22:1541-1549.
