Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Bovine,Canine,Chicken,Mouse,Non-humanprimate,Rat
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- SLC32A1
- MolecularReference:
- ~53kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2100-VGAT,RRID:AB_2492282
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionofratVesicularGABAAminoAcidTransporter(VGAT).
Theantibodyispreparedfromrabbitserumbyaffinitypurificationonacolumnmadewiththepeptideusedasantigen.
BIOLOGicalSignificance:Collapse
TheVesicularGABAAminoAcidTransporter(VGAT)isresponsIBLefortransportoftheinhibitoryneurotransmitterintosynapticvesicles(McIntireetal.,1997).TheVGATprotein(alsoknownastheVesicularInhibitoryAminoAidTransporterorVIAAT)isexpressedinsynapticvesiclesofbothglycineandGABAergicsynapsesthroughouttheCNS(Chaudhryetal.,1998).ExpressionoftheVGATproteinchangesduringdevelopmentandalsoinresponsetopatternsofneuronalactivity(Deetal.,2005).
Storage
Liquid.Contains10mMsodiumazide.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
ProductSpecificReferences
Ka,M.,Kook,Y.H.,Liao,K.,Buch,S.andKim,W.Y.,2016.TransactivationofTrkBbySigma-1receptormediatescocaine-inducedchangesindendriticspinedensityandmorphologyinhippocampalandcorticalneurons.CellDeath&Disease,7(10),p.e2414.PMID:27735948
Ka,M.,Chopra,D.A.,Dravid,S.M.,&Kim,W.Y.(2016).EssentialRolesforARID1BinDendriticArborizationandSpineMorphologyofDevelopingPyramidalNeurons.TheJournalofNeuroscience,36(9),2723-2742.PMID:26937011
Rotschafer,S.E.,Marshak,S.,&Cramer,K.S.(2015).DeletionofFmr1AltersFunctionandSynapticInputsintheAuditoryBrainstem.PloSone,Feb13;10(2):e0117266.PMID:25679778
Noseda,R.,Kainz,V.,Borsook,D.,&Burstein,R.(2014).NeuRochemicalpathwaysthatconvergeonthalamictrigeminovascularneurons:potentialsubstrateformodulationofmigrainebysleep,foodintake,stressandanxiety.PLoSOne.Aug4;9(8):e103929.PMID:25090640
