Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat
- Applications:
- WB1:1,000IHC1:500
- Species:
- Rabbit
- GeneName:
- GRM1
- MolecularReference:
- ~125kDaand~250kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2031-mGluR1a,RRID:AB_2492150
Antigen/Purification:Collapse
TheantigenisapeptidefromtheC-terminalregionofratmGluR1a.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Themetabotropicglutamatereceptors(mGluRs)arekeyreceptorsinthemodulationofexcitatorysynaptictransmissioninthecentralnervoussystem.Theyareimplicatedinmanyformsofneuralplasticityaswellaslearningandmemoryanddrugabuse(Bhattacharyaetal.,2004;Francesconietal.,2004;WilsonandNicoll,2001).GroupImetabotropicglutamatereceptors(consistingofmGluR1andmGluR5)areG-protein-coupledneurotransmitterreceptorsthatarelocalizedintheperisynapticregionofthepostsynapticmembrane.Whenactivated,GroupImGluRsleadtostimulationofphospholipaseandactivationofProteinKinaseC.Incontrast,activationofGroupIImetabotropicreceptors(mGluR2andmGluR3)leadstoinhibitionofadenylatecyclase.ThemGluR1receptormayalsobecriticallyinvolvedinlimitingthedeleteriouseffectsofexcitotoxicity(Blaabjergetal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternblots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
BhattacharyaM,BabwahAV,GodinC,AnborghPH,DaleLB,PoulterMO,FergusonSSG(2004)RalandphospholipaseD2-dependentpathwayforconstitutivemetabotropicglutamatereceptorendocytosis.JNeurosci24:8752-8761.
BlaabjergM,FangLW,ZimmerJ,BaskysA(2003)NeuroprotectionagainstNMDAexcitotoxicitybygroupImetabotropicglutamatereceptorsisassociatedwithreductionofNMDAstimulatedcurrents.ExpNeurol183:573-580.
FrancesconiW,CammalleriM,SannaPP(2004)Themetabotropicglutamatereceptor5isnecessaryforlate-phaselong-termpotentiationinthehippocampalCA1region.BrainRes1022:12-18.
WilsonRI,NicollRA(2001)EndogenouscannABInoidsmediateretrogradesignallingathippocampalsynapses.Nature(London)410:588-592.
