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Functional_antibody >
PhosphoSolutions/Metabotropic Glutamate Receptor 2/3 Antibody/2030-mGluR2/3/100 µl
Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Affinitypurified
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000IHC1:500
- Species:
- Rabbit
- GeneName:
- GRM2/3
- MolecularReference:
- ~110kDaand~220kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#2030-mGluR2/3,RRID:AB_2492151
Antigen/Purification:Collapse
TheantigenisapeptidefromtheC-terminalregionofratmGluR2andratmGluR3.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Themetabotropicglutamatereceptors(mGluRs)arekeyreceptorsinthemodulationofexcitatorysynaptictransmissioninthecentralnervoussystem.Theyareimplicatedinmanyformsofneuralplasticityaswellaslearningandmemoryanddrugabuse(Bhattacharyaetal.,2004;Francesconietal.,2004;WilsonandNicoll,2001).GroupImetabotropicglutamatereceptors(consistingofmGluR1andmGluR5)areG-protein-coupledneurotransmitterreceptorsthatarelocalizedintheperisynapticregionofthepostsynapticmembrane.Whenactivated,GroupImGluRsleadtostimulationofphospholipaseandactivationofProteinKinaseC.Incontrast,activationofGroupIImetabotropicreceptors(mGluR2andmGluR3)leadstoinhibitionofadenylatecyclase.ThemGluR2subunithasbeenshowntoberequiredforlong-termpotentiationatthemossyfiberinputinthehippocampus(Yokoietal.,1996).
Storage
100µlin200mMTris-Glycine(pH7.5),0.1%sodiumazide.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
