Mousemonoclonalantibody
- Size:
- 15µg
- Formulation:
- Lyophilizedfromculturesupernatant
- Specificity:
- Rat,Mouse
- Applications:
- WB1:1,000IP3µgper200µglysate
- Species:
- Mouse
- GeneName:
- GRIN1
- MolecularReference:
- ~120kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1508-NR1,RRID:AB_2492165
Antigen/Purification:Collapse
Theantigenisafusionproteincontainingaminoacids1-564oftheNR1subunitofratNMDAreceptor.
Theantibodyisculturesupernatant.
BIOLOGicalSignificance:Collapse
Theionchannelsactivatedbyglutamatearetypicallydividedintotwoclasses.Glutamatereceptorsthatareactivatedbykainateandα-amino-3-hydroxy-5-methyl-4-isoxalonepropionicacid(AMPA)areknownaskainate/AMPAreceptors(K/AMPAR).ThosethataresensitivetoNmethyl-D-aspartate(NMDA)aredesignatedNMDAreceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheNMDAreceptorisalsooneoftheprincipalmoleculartargetsforalcoholintheCNS(Lovingeretal.,1989;Alvestadetal.,2003;Snelletal.,1996).TheNMDARisalsopotentiatedbyproteinphosphorylation(Luetal.,1999).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1-subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits.
Storage
15µgantibodyvial;lyophilizedfromculturesupernatant.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Ando,Y.,Shimazaki,H.,Shiota,K.,Tetsuka,S.,Nakao,K.,Shimada,T.,Kurata,K.,Kuroda,J.,Yamashita,A.,Sato,H.andSato,M.(2016).Prevalenceofelevatedserumanti-N-methyl-D-aspartatereceptorantibodytitersinpatientspresentingexclusivelywithpsychiatricsymptoms:acomparativefollow-upstudy.BMCpsychiatry,16(1),226.PMID:27391811
Quillinan,N.,Grewal,H.,Deng,G.,Shimizu,K.,Yonchek,J.C.,Strnad,F.,TraystmanR.J.,&Herson,P.S.(2015).Region-specificroleforGluN2B-containingNMDAreceptorsininjurytoPurkinjecellsandCA1neuronsfollowingglobalcerebralischemia.Neuroscience,284,555-565.PMID:25450957
HicklinTR,WuPH,RadcliffeRA,FreundRK,Goebel-GoodySM,CorreraPR,ProctorWR,LombrosoPJ,andBrowningMD(2011)AlcoholinhibitionoftheNMDAreceptorfunction,long-termpotentiation,andfearlearningrequiresstriatal-enrichedproteintyrosinephosphatase.ProcNatlAcadSciUSA.108(16):6650-5.PMID:21464302
KurtisD.Davies,SusanM.Goebel-Goody,StevenJ.Coultrap,andMichaelD.Browning(2008)LongTermSynapticDepressionThatIsAssociatedwithGluR1DephosphorylationbutNot-Amino-3-hydroxy-5-methyl-4-isoxazolepropionicAcid(AMPA)ReceptorInternalizationJ.Biol.Chem.,283:33138–33146.PMID:18819923
GuohuaZhang,YuanlinDong,BinZhang,FumitoIchinose,XuWu,DeborahJ.Culley,GregoryCrosby,RudolphE.Tanzi,andZhongcongXie(2008)Isoflurane-InducedCaspase-3ActivationIsDependentonCytosolicCalciumandCanBeAttenuatedbyMemantineJ.Neurosci.,4551–4560.PMID:18434534
