Rabbitpolyclonalantibody
PooledSerum- Size:
- 25µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000IHC1:1,000to1:2,000
- Species:
- Rabbit
- GeneName:
- GRIN1
- MolecularReference:
- ~120kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1505-C1,RRID:AB_2492166
Antigen/Purification:Collapse
TheantigenisapeptidefromtheNR1subunit,C1splicevariantinsertoftheNMDAreceptor.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
TheionchannelsactivatedbyglutamatethataresensitivetoN-methyl-Daspartate(NMDA)aredesignatedNMDAReceptors(NMDAR).TheNMDARplaysanessentialroleinmemoryandneuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).ThereareanumberofdifferentsplicevariantsoftheNR1-Subunit(Foldesetal.,1994;ZukinandBennett,1995).DifferentialsplicingofthreeexonsintheNR1-SubunitgeneratesuptoeightNR1-Subunitsplicevariantsand7ofthesehavebeenidentifiedinCDNAlibraries.Theseexonsencodea21aminoacidN-terminaldomain(N1)andadjacentsequencesintheC-terminus(C1andC2).SplicingouttheC2cassetteeliminatesthefirststopcodonandproducesanewreADIngframethatgeneratesanewsequenceof22aminoacids(C2′).ConsiderableattentionhasbeenfocusedonthedistributionandexpressionofthesesplicevariantsthatmayaffectthefunctionalpropertiesandregulationoftheNMDAR
Storage
25µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
BradyML,DiazMR,IusoA,EverettJC,ValenzuelaCF,CaldwellKK(2013)ModerateprenatalalcoholexposurereducesplasticityandaltersNMDAreceptorsubunitcompositioninthedentategyrus.JNeurosci.33(3):1062-7.PMID:23325244
