Rabbitpolyclonalantibody
PooledSerum- Size:
- 25µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000IHC1:1,000to1:2,000
- Species:
- Rabbit
- GeneName:
- GRIN1
- MolecularReference:
- ~120kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1506-C2,RRID:AB_2492167
Antigen/Purification:Collapse
TheantigenisapeptidefromtheNR1subunit,C2splicevariantinsertofratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
TheionchannelsactivatedbyglutamatethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAReceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).ThereareanumberofdifferentsplicevariantsoftheNR1subunit(Foldesetal.,1994;ZukinandBennett,1995).DifferentialsplicingofthreeexonsintheNR1subunitgeneratesuptoeightNR1subunitsplicevariantsand7ofthesehavebeenidentifiedinCDNAlibraries.Theseexonsencodea21aminoacidN-terminaldomain(N1)andadjacentsequencesintheC-terminus(C1andC2).SplicingouttheC2cassetteeliminatesthefirststopcodonandproducesanewreADIngframethatgeneratesanewsequenceof22aminoacids(C2′).ConsiderableattentionhasbeenfocusedonthedistributionandexpressionofthesesplicevariantsthatmayaffectthefunctionalpropertiesandregulationoftheNMDAR.
Storage
25µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
BradyML,DiazMR,IusoA,EverettJC,ValenzuelaCF,CaldwellKK(2013)ModerateprenatalalcoholexposurereducesplasticityandaltersNMDAreceptorsubunitcompositioninthedentategyrus.JNeurosci.33(3):1062-7. PMID:23325244
MannieM.Y.Fan,HermanB.Fernandes,LilyY.J.Zhang,MichaelR.Hayden,andLynnA.Raymond(2007)AlteredNMDAReceptorTraffickinginaYeastArtificialChromosomeTransgenicMouseModelofHuntington’sDiseaseJ.Neurosci.,27:3768–3779. PMID:17409241
