Rabbitpolyclonalantibody
PooledSerum- Size:
- 10µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000IHC1:1,000to1:2,000IP3µlper200µglysate
- Species:
- Rabbit
- GeneName:
- GRIN2A
- MolecularReference:
- ~180kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1500-NR2A,RRID:AB_2492170
Antigen/Purification:Collapse
TheantigenisafusionproteinfromtheC-terminusoftheNR2AsubunitofratNMDAreceptor.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Theionchannelsactivatedbyglutamatearetypicallydividedintotwoclasses.Glutamatereceptorsthatareactivatedbykainateandα-amino-3-hydroxy-5-methyl-4-isoxalonepropionicacid(AMPA)areknownaskainate/AMPAreceptors(K/AMPAR).ThosethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAreceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheNMDAreceptorisalsooneoftheprincipalmoleculartargetsforalcoholintheCNS(Lovingeretal.,1989;Alvestadetal.,2003;Snelletal.,1996).TheNMDARisalsopotentiatedbyproteinphosphorylation(Luetal.,1999).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits.
Storage
10µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Goodfellow,M.J.,ABDulla,K.A.,&Lindquist,D.H.(2016).NeonatalethanolexposureimpairstracefearconditioningandaltersNMDAreceptorsubunitexpressioninadultmaleandfemalerats.Alcoholism:ClinicalandExperimentalResearch,40(2),309-318.PMID:26842250
O’Leary,H.,Bernard,P.B.,Castano,A.M.,&Benke,T.A.(2016).EnhancedlongtermpotentiationanddecreasedAMPAreceptordesensitizationintheacuteperiodfollowingasinglekainateinducedearlylifeseizure.Neurobiologyofdisease,87,134-144.PMID:26706598
Beshara,S.,Beston,B.R.,Pinto,J.G.,&Murphy,K.M.(2015).EffectsoffluoxetineandvisualexperienceonglutamatergicandGABAergicsynapticproteinsinadultratvisualcortex.eneuro,ENEURO-0126.PMID:26730408
Bostrom,C.A.,Majaess,N.M.,Morch,K.,White,E.,&Christie,B.R.(2013).RescueofNMDAR-dependentsynapticplasticityinFmr1knock-outmice.Cerebralcortex,bht237.PMID:23968838
Pozniak,C.D.,Ghosh,A.S.,Gogineni,A.,Hanson,J.E.,Lee,S.H.,Larson,J.L.,Solanoy,H.,Bustos,D.,Li,H.,Ngu,H.&Jubb,A.M.(2013).Dualleucinezipperkinaseisrequiredforexcitotoxicity-inducedneuronaldegeneration.TheJournalofexperimentalmedicine,210(12),2553-2567.PMID: 24166713
Priya,A.,Johar,K.,&Wong-Riley,M.T.(2013).Specificityprotein4functionallyregulatesthetranscriptionofNMDAreceptorsubunitsGluN1,GluN2A,andGluN2B.BiochimicaetBiophysicaActa(BBA)-MolecularCellResearch,1833(12),2745-2756.PMID:23871830
HicklinTR,WuPH,RadcliffeRA,FreundRK,Goebel-GoodySM,CorreraPR,ProctorWR,LombrosoPJ,andBrowningMD.(2011)AlcoholinhibitionoftheNMDAreceptorfunction,long-termpotentiation,andfearlearningrequiresstriatal-enrichedproteintyrosinephosphatase.ProcNatlAcadSciUSA108(16):6650-5.PMID:21464302
AlvestadRM,GrosshansDR,CoultrapSJ,NakazawaT,YamamotoT,BrowningMD(2003)TyrosinedephosphorylationandethanolinhibitionofN-methyl-D-aspartatereceptorfunction.JBiolChem278:11020-11025.PMID:12536146
WentholdRJ,PrybylowskiK,StandleyS,SansN,PetraliaRS(2003)TraffickingofNMDAreceptors.AnnuRevPharmacolToxicol43:335-358.PMID:12540744
GrosshansDR,ClaytonDA,CoultrapSJ,BrowningMD(2002)LTPleadstorapidsurfaceexpressionofNMDAbutnotAMPAreceptorsinadultratCA1.NatNeurosci5:27-33.PMID:11740502
