>
产品中心 >
Functional_antibody >
PhosphoSolutions/NMDA Receptor NR2A Subunit N-terminus Antibody/1501-NR2A/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Bovine,Canine
- Applications:
- WB1:1,000IHC1:500
- Species:
- Rabbit
- GeneName:
- GRIN2A
- MolecularReference:
- ~180kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1501-NR2A,RRID:AB_2492173
Antigen/Purification:Collapse
TheantigenisapeptidefromtheN-terminusoftheNR2AsubunitofratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
Theionchannelsactivatedbyglutamatearetypicallydividedintotwoclasses.Glutamatereceptorsthatareactivatedbykainateandα-amino-3-hydroxy-5-methyl-4-isoxalonepropionicacid(AMPA)areknownaskainate/AMPAreceptors(K/AMPAR).ThosethataresensitivetoN-methyl-D-aspartate(NMDA)aredesignatedNMDAreceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheNMDAreceptorisalsooneoftheprincipalmoleculartargetsforalcoholintheCNS(Lovingeretal.,1989;Alvestadetal.,2003;Snelletal.,1996).TheNMDARisalsopotentiatedbyproteinphosphorylation(Luetal.,1999).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits.
Storage
100μlantibodyvialin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
AlvestadRM,GrosshansDR,CoultrapSJ,NakazawaT,YamamotoT,BrowningMD(2003)TyrosinedephosphorylationandethanolinhibitionofN-methyl-D-aspartatereceptorfunction.JBiolChem278:11020-11025.
CarrollRC,ZukinRS(2002)NMDA-receptortraffickingandtargeting:implicationsforsynaptictransmissionandplasticity.TrendsNeurosci25:571-577.
GrosshansDR,ClaytonDA,CoultrapSJ,BrowningMD(2002)LTPleadstorapidsurfaceexpressionofNMDAbutnotAMPAreceptorsinadultratCA1.NatNeurosci5:27-33.
LovingerDM,WhiteG,WeightFF(1989)EthanolinhibitsNMDA-activatedioncurrentinhippocampalneurons.Science243:1721-1724.
LuW-Y,XiongZ-G,LeiS,OrserBA,BrowningMD,MacDonaldJF(1999)G-proteincoupledreceptorsactviaproteinkinaseCandSrctoregulateNMDAreceptors.NatureNeurosci2:331-338.
SnellLD,NunleyKR,LickteigRL,BrowningMD,TabakoffB,HoffmanPL(1996)RegionalandsubunitspecificchangesinNMDAreceptormRNAandimmunoreactivityinmousebrainfollowingchronicethanolingestion.MolBrainRes40:71-78.
WentholdRJ,PrybylowskiK,StandleyS,SansN,PetraliaRS(2003)TraffickingofNMDAreceptors.AnnuRevPharmacolToxicol43:335-358.
Note:Dr.MichaelBrowning,aco-authoroffourofthecitedpapers,isCEOandfounderofPhosphoSolutions.
