>
产品中心 >
Functional_antibody >
PhosphoSolutions/NMDA Receptor NR2B Subunit (Ser1166) Antibody/p1516-1166/100 µl
Rabbit polyclonal antibody
Pooled Serum- Size:
- 100 µl
- Formulation:
- Affinity Purified from Pooled Serum
- Specificity:
- Rat, Mouse, Human, Xenopus, Non-human primate, Canine
- Applications:
- WB 1:250
- Species:
- Rabbit
- Gene Name:
- GRIN2B
- Molecular Reference:
- ~180 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1516-1166, RRID:AB_2492179
Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser1166 of the NR2B subunit of the rat NMDA receptor.
The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: Collapse
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Phosphorylation of Ser1166 is thought to play an essential role in memory and neuronal development.
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Tassin, T. C., Benavides, D. R., Plattner, F., Nishi, A., & Bibb, J. A. (2015). Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain. Journal of Biological Chemistry, jbc-M115. PMID: 25971971
Murphy, J. A., Stein, I. S., Lau, C. G., Peixoto, R. T., Aman, T. K., Kaneko, N., Aromolaran, K., Saulnier, J.L., Popescu, G.K., Sabatini, B.L., Hell, J.W., & Zukin, R. S. (2014). Phosphorylation of Ser1166 on GluN2B by PKA is critical to synaptic NMDA receptor function and Ca2+ signaling in spines. The Journal of Neuroscience, 34(3), 869-879. PMID: 24431445
