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Functional_antibody >
PhosphoSolutions/NMDA Receptor NR2B Subunit (Ser1480) Antibody/p1516-1480/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- GRIN2B
- MolecularReference:
- ~180kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1516-1480,RRID:AB_2492183
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser1480oftheNR2BsubunitoftheratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
TheNMDAreceptor(NMDAR)playsanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits(Ishiietal.,1993).IthasbeenshownthatphosphorylationofSer1480 disruptstheinteractionofNR2BwiththePDZdomainsofPSD-95andSAP102anddecreasessurfaceNR2Bexpressioninneurons(Chungetal.,2004).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Zamzow,D.R.,Elias,V.,Acosta,V.A.,Escobedo,E.,&Magnusson,K.R.(2016).HigherlevelsofphosphorylatedY1472onGluN2Bsubunitsinthefrontalcortexofagedmiceareassociatedwithgoodspatialreferencememory,butnotcognitiveflexibility.AGE,38(3),1-17.PMID:27094400
