>
产品中心 >
Functional_antibody >
PhosphoSolutions/NMDA Receptor NR2B Subunit (Tyr1252) Antibody/p1516-1252/100 µl
Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1000IHC1:400
- Species:
- Rabbit
- GeneName:
- GRIN2B
- MolecularReference:
- ~180kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1516-1252,RRID:AB_2492180
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Tyr1252oftheNR2BsubunitoftheratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
TheNMDAreceptor(NMDAR)playsanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits(Ishiietal.,1993).PhosphorylationofTyr1252isthoughttopotentiateNMDAreceptor-dependentinfluxofcalcium(Takasuetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
TiannaR.Hicklin,PeterH.Wu,RichardA.Radcliffe,RonaldK.Freund,SusanM.Goebel-Goody,PauloR.Correa,WilliamR.Proctor,PaulJ.Lombroso,andMichaelD.Browning(2011)AlcoholinhibitionoftheNMDAreceptorfunction,long-termpotentiation,andfearlearningrequiresstriatal-enrichedproteintyrosinephosphatasePNAS,Apr2011;108:6650–6655.PMID:21464302
