Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate
- Applications:
- WB1:1000IHC1:400
- Species:
- Rabbit
- GeneName:
- GRIN2B
- MolecularReference:
- ~180kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1516-1336,RRID:AB_2492181
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Tyr1336oftheNR2BsubunitoftheratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits(Ishiietal.,1993).PhosphorylationofTyr1336isthoughttopotentiateNMDAreceptor-dependentinfluxofcalcium(Takasuetal.,2002)andischemiamayalsoincreasethephosphorylationofthissite(Takagietal.,2003).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Holmes,A.,Zhou,N.,Donahue,D.L.,Balsara,R.andCastellino,F.J.,2017.AdeficiencyoftheGluN2CsubunitoftheN-Methyl-d-Aspartatereceptorisneuroprotectiveinamousemodelofischemicstroke.BiochemicalandBiophysicalResearchCommunications.PMID:29101031
Zamzow,D.R.,Elias,V.,Acosta,V.A.,Escobedo,E.,&Magnusson,K.R.,2016.HigherlevelsofphosphorylatedY1472onGluN2Bsubunitsinthefrontalcortexofagedmiceareassociatedwithgoodspatialreferencememory,butnotcognitiveflexibility.AGE,38(3),1-17.PMID:27094400
TiannaR.Hicklin,PeterH.Wu,RichardA.Radcliffe,RonaldK.Freund,SusanM.Goebel-Goody,PauloR.Correa,WilliamR.Proctor,PaulJ.Lombroso,andMichaelD.Browning(2011)AlcoholinhibitionoftheNMDAreceptorfunction,long-termpotentiation,andfearlearningrequiresstriatal-enrichedproteintyrosinephosphatasePNAS,Apr2011;108:6650–6655.PMID:21464302
TatyanaChernova,JoernR.Steinert,ChristopherJ.Guerin,PierluigiNicotera,IanD.Forsythe,andAndrewG.Smith(2007)NeuriteDegenerationInducedbyHemeDeficiencyMediatedviaInhibitionofNMDAReceptor-DependentExtracellularSignal-RegulatedKinase1/2Activation.J.Neurosci.,27:8475–8485.PMID:17687025
