Rabbit polyclonal antibody
Pooled Serum- Size:
- 100 µl
- Formulation:
- Affinity Purified from Pooled Serum
- Specificity:
- Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Zebra fish, Canine
- Applications:
- WB 1:1,000
- Species:
- Rabbit
- Gene Name:
- GRIN2B
- Molecular Reference:
- ~180 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1516-1472, RRID:AB_2492182
Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1472 of NMDA NR2B.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: Collapse
The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity (Nakazawa et al., 2001; Roche et al., 2001).
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Jang, S. S., Royston, S. E., Lee, G., Wang, S., & Chung, H. J. (2016). Seizure-Induced Regulations of Amyloid-β, STEP61, and STEP61 Substrates Involved in Hippocampal Synaptic Plasticity. Neural plasticity, 2016. PMID: 27127657
Xiao, X., Levy, A. D., Rosenberg, B. J., Higley, M. J., & Koleske, A. J. (2016). Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 36(25), 6778-6791. PMID: 27335408
Zamzow, D. R., Elias, V., Acosta, V. A., Escobedo, E., & Magnusson, K. R. (2016). Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17. PMID: 27094400
Jang, S. S., Royston, S. E., Xu, J., Cavaretta, J. P., Vest, M. O., Lee, K. Y., Lee, S., Jeong, H.G., Lombroso, P., & Chung, H. J. (2015). Regulation of STEP61 and tyrosine-phosphorylation of NMDA and AMPA receptors during homeostatic synaptic plasticity. Molecular brain, 8. PMID: 26391783
Mao, L. M., & Wang, J. Q. (2015). Dopaminergic and cholinergic regulation of Fyn tyrosine kinase phosphorylation in the rat striatum in vivo. Neuropharmacology, 99, 491-499. PMID: 26277342
Chen, W., Walwyn, W., Ennes, H. S., Kim, H., McRoberts, J. A., & Marvizón, J. C. G. (2014). BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals. European Journal of Neuroscience, 39(9), 1439-1454. PMID: 24611998
Knox, R., Brennan-Minnella, A. M., Lu, F., Yang, D., Nakazawa, T., Yamamoto, T., Swanson, R.A., Ferriero, D.M., & Jiang, X. (2014). NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia. Stroke, 45(10), 3040-3047. PMID: 25158771
Gladding CM, Sepers MD, Xu J, Zhang LY, Milnerwood AJ, Lombroso PJ, Raymond LA (2012) Calpain and Striatal-Enriched protein tyrosine phosphatase (STEP) activation contribute to extrasynaptic NMDA receptor localization in a Huntington’s disease mouse model. Hum Mol Genet. Sep 1;21(17):3739-52. PMID: 22523092
Xu J, Kurup P, Bartos JA, Patriarchi T, Hell JW, Lombroso PJ (2012) Striatal-enriched protein-tyrosine phosphatase (STEP) regulates Pyk2 kinase activity. J Biol Chem Jun 15;287(25):20942-56. PMID: 22544749
Tianna R. Hicklin, Peter H. Wu, Richard A. Radcliffe, Ronald K. Freund, Susan M. Goebel-Goody, Paulo R. Correa, William R. Proctor, Paul J. Lombroso, and Michael D. Browning (2011) Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase PNAS, Apr 2011; 108: 6650 – 6655. PMID: 21464302
