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PhosphoSolutions/NMDA Receptor NR2B Subunit Antibody, 10 µg/1499-NR2B/10 µg
Rabbitpolyclonalantibody
PooledSerum- Size:
- 10µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Bovine,Canine
- Applications:
- WB1:1,000IHC1:1,000to1:2,000IP3µlper200µglysate
- Species:
- Rabbit
- GeneName:
- GRIN2B
- MolecularReference:
- ~180kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1499-NR2B,RRID:AB_2492175
Antigen/Purification:Collapse
TheantigenisafusionproteinfromtheC-terminusoftheNR2BsubunitofratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
TheionchannelsactivatedbyglutamatethataresensitivetoN-methyl-Daspartate(NMDA)aredesignatedNMDAreceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheNMDAreceptorisalsooneoftheprincipalmoleculartargetsforalcoholintheCNS(Lovingeretal.,1989;Alvestadetal.,2003;Snelletal.,1996).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobeclonedanditcanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits.OverexpressionoftheNR2B-subunitoftheNMDAreceptorhasbeenassociatedwithincreasesinlearningandmemorywhileaged,memoryimpairedanimalshavedeficienciesinNR2Bexpression(Claytonetal.,2002a;Claytonetal.,2002b).TheNMDARisalsopotentiatedbyproteinphosphorylation(Luetal.,1999).
Storage
10µgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
O’Leary,H.,Bernard,P.B.,Castano,A.M.,&Benke,T.A.(2016).EnhancedlongtermpotentiationanddecreasedAMPAreceptordesensitizationintheacuteperiodfollowingasinglekainateinducedearlylifeseizure.Neurobiologyofdisease,87,134-144.PMID:26706598
Bostrom,C.A.,Majaess,N.M.,Morch,K.,White,E.,&Christie,B.R.(2013).RescueofNMDAR-dependentsynapticplasticityinFmr1knock-outmice.Cerebralcortex,bht237.PMID:23968838
MeganL.Brady,MarvinR.Diaz,AnthonyIuso,JulieC.Everett,C.FernandoValenzuela,andKevinK.Caldwell.(2013)ModerateprenatalalcoholexposurereducesplasticityandaltersNMDAreceptorsubunitcompositioninthedentategyrus.JNeourosci.33(3):1062-7.PMID:23325244
TiannaR.Hicklin,PeterH.Wu,RichardA.Radcliffe,RonaldK.Freund,SusanM.Goebel-Goody,PauloR.Correa,WilliamR.Proctor,PaulJ.Lombroso,andMichaelD.Browning(2011)AlcoholinhibitionoftheNMDAreceptorfunction,long-termpotentiation,andfearlearningrequiresstriatal-enrichedproteintyrosinephosphatasePNAS,Apr2011;108:6650–6655.PMID:21464302
PradeepKurup,YongfangZhang,JianXu,DeepaV.Venkitaramani,VahramHaroutunian,PaulGreengard,AngusC.Nairn,andPaulJ.Lombroso(2010)A-beta-MediatedNMDAReceptorEndocytosisinAlzheimer’sDiseaseInvolvesUbiquitinationoftheTyrosinePhosphataseSTEP61.J.Neurosci.,30:5948–5957.PMID:20427654
JianXu,PradeepKurup,YongfangZhang,SusanM.Goebel-Goody,PeterH.Wu,AmmarH.Hawasli,MatthewL.Baum,JamesA.Bibb,andPaulJ.LombrosoExtrasynapticNMDAReceptorsCouplePreferentiallytoExcitotoxicityviaCalpain-MediatedCleavageofSTEP(2009)J.Neurosci.,29:9330–9343.PMID:19625523
KurtisD.Davies,SusanM.Goebel-Goody,StevenJ.Coultrap,andMichaelD.Browning(2008)LongTermSynapticDepressionThatIsAssociatedwithGluR1DephosphorylationbutNotalpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionicAcid(AMPA)ReceptorInternalizationJ.Biol.Chem.,283:33138–33146.PMID:18819923
ErinH.NorrisandSidneyStrickland(2007)ModulationofNR2B-regulatedcontextualfearinthehippocampusbythetissueplasminogenactivatorsystem.PNAS,104:13473-13478.PMID:17673549
TatyanaChernova,JoernR.Steinert,ChristopherJ.Guerin,PierluigiNicotera,IanD.Forsythe,andAndrewG.Smith(2007)NeuriteDegenerationInducedbyHemeDeficiencyMediatedviaInhibitionofNMDAReceptor-DependentExtracellularSignal-RegulatedKinase1/2ActivationJ.Neurosci.,27:8475–8485.PMID:17687025
