Rabbit polyclonal antibody
Pooled Serum- Size:
- 50 µl
- Formulation:
- Sera pooled, neat serum
- Specificity:
- Rat, Mouse, Human
- Applications:
- WB 1:1,000
- Species:
- Rabbit
- Gene Name:
- GRIN2B
- Molecular Reference:
- ~180 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 1498-NR2B, RRID:AB_2492177
Antigen/Purification: Collapse
The antigen is a fusion protein from the C-terminus region of the NR2B subunit of rat NMDA receptor.
The antibody is pooled, unpurified neat serum.
Biological Significance: Collapse
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Storage
50 μl neat serum. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Schwab, A.J., Sison, S.L., Meade, M.R., Broniowska, K.A., Corbett, J.A. and Ebert, A.D., 2017. Decreased Sirtuin Deacetylase Activity in LRRK2 G2019S iPSC-Derived Dopaminergic Neurons. Stem Cell Reports, Vol 9, 1-14, Dec 12. PMID: 29129681
Priya, A., Johar, K., & Wong-Riley, M. T. (2013). Specificity protein 4 functionally regulates the transcription of NMDA receptor subunits GluN1, GluN2A, and GluN2B. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1833(12), 2745-2756. PMID: 23871830
