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PhosphoSolutions/NMDA Receptor NR2C Subunit Antibody/1502-NR2C/10 µg
Rabbitpolyclonalantibody
PooledSerum- Size:
- 10µg
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000IP3µlper200µglysate
- Species:
- Rabbit
- GeneName:
- GRIN2C
- MolecularReference:
- ~140kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1502-NR2C,RRID:AB_2492184
Antigen/Purification:Collapse
TheantigenisafusionproteinfromtheN-terminusoftheNR2C-subunitofratNMDAreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
TheionchannelsactivatedbyglutamatethataresensitivetoN-methyl-Daspartate(NMDA)aredesignatedNMDAreceptors(NMDAR).TheNMDARplaysanessentialroleinmemory,neuronaldevelopmentandithasalsobeenimplicatedinseveraldisordersofthecentralnervoussystemincludingAlzheimer’s,epilepsyandischemicneuronalcelldeath(Grosshansetal.,2002;Wentholdetal.,2003;CarrollandZukin,2002).TheNMDAreceptorisalsooneoftheprincipalmoleculartargetsforalcoholintheCNS(Lovingeretal.,1989;Alvestadetal.,2003;Snelletal.,1996).TheNMDARisalsopotentiatedbyproteinphosphorylation(Luetal.,1999).TheratNMDAR1(NR1)wasthefirstsubunitoftheNMDARtobecloned.TheNR1proteincanformNMDAactivatedchannelswhenexpressedinXenopusoocytesbutthecurrentsinsuchchannelsaremuchsmallerthanthoseseeninsitu.ChannelswithmorephysiologicalcharacteristicsareproducedwhentheNR1subunitiscombinedwithoneormoreoftheNMDAR2(NR2A-D)subunits.TheNR2CsubunitofthereceptoristhoughttoinfluencetheNMDARconductancelevel(Ebralidzeetal.,1996).
Storage
10μgantibodyvial;lyophilizedfrom5mMammoniumbicarbonate.Theantibodyshouldbereconstitutedin50µlphosphatebufferedsaline(PBS:137mMNaCl,7.5mMNa2HPO4,2.7mMKCl,1.5mMKH2PO4,pH7.4)beforeuse.Afterreconstitutiontheantibodyshouldbealiquotedandstoredat-20°C.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
AlvestadRM,GrosshansDR,CoultrapSJ,NakazawaT,YamamotoT,BrowningMD(2003)TyrosinedephosphorylationandethanolinhibitionofN-methyl-D-aspartatereceptorfunction.JBiolChem278:11020-11025.
CarrollRC,ZukinRS(2002)NMDA-receptortraffickingandtargeting:implicationsforsynaptictransmissionandplasticity.TrendsNeurosci25:571-577.
GrosshansDR,ClaytonDA,CoultrapSJ,BrowningMD(2002)LTPleadstorapidsurfaceexpressionofNMDAbutnotAMPAreceptorsinadultratCA1.NatNeurosci5:27-33.
LovingerDM,WhiteG,WeightFF(1989)EthanolinhibitsNMDA-activatedioncurrentinhippocampalneurons.Science243:1721-1724.
LuW-Y,XiongZ-G,LeiS,OrserBA,BrowningMD,MacDonaldJF(1999)G-proteincoupledreceptorsactviaproteinkinaseCandSrctoregulateNMDAreceptors.NatureNeurosci2:331-338.
SnellLD,NunleyKR,LickteigRL,BrowningMD,TabakoffB,HoffmanPL(1996)RegionalandsubunitspecificchangesinNMDAreceptormRNAandimmunoreactivityinmousebrainfollowingchronicethanolingestion.MolBrainRes40:71-78.
WentholdRJ,PrybylowskiK,StandleyS,SansN,PetraliaRS(2003)TraffickingofNMDAreceptors.AnnuRevPharmacolToxicol43:335-358.
Note:Dr.MichaelBrowning,aco-authoroffourofthecitedpapers,isPresidentandfounderofPhosphoSolutions.
