Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~135kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1551-940,RRID:AB_2492213
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser940ofratKCC2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-andnon-phosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
KCC2iswidelythoughttobeexpressedexclusivelyinneuronswhereitisresponsIBLeformaintaininglowintracellularchlorideconcentrationtodrivehyperpolarizingpost-synapticresponsestotheinhibitoryneurotransmittersGABAandglycine.Serine940onKCC2hasbeenshowntobephosphorylatedbyPKCandhasfurtherbeendemonstratedtobethemajorsiteforPKC-dependentphosphorylationforfulllengthKCC2moleculeswhenexpressedinHEK-293cellsasphosphorylationofSer940increasedthecellsurfacestABIlityofKCC2inthissystembydecreasingit’srateofinternalizationfromtheplasmamembrane(Leeetal.,2007).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Product SpecificReferences
Conway,L.C.,Cardarelli,R.A.,Moore,Y.E.,Jones,K.,McWilliams,L.J.,Baker,D.J.,Burnham,M.P.,Bürli,R.W.,Wang,Q.,Brandon,N.J.andMoss,S.J.,2017.N-ethylmaleimideincreasesKCC2activitybymodulatingtransporterphosphorylation.JournalofBiologicalChemistry,pp.jbc-M117.PMID:29092909
Rinetti-Vargas,G.,Phamluong,K.,Ron,D.andBender,K.J.,2017.PeriadolescentMaturationofGABAergicHyperpolarizationattheAxonInitialSegment.CellReports,20(1),pp.21-29.PMID:28683314
Huang,Y.J.,Lee,K.H.,Murphy,L.,Garraway,S.M.andGrau,J.W.,2016.Acutespinalcordinjury(SCI)transformshowGABAaffectsnociceptivesensitization.ExperimentalNeurology,285,pp.82-95.PMID:27639636
López-Álvarez,V.M.,Modol,L.,Navarro,X.,Cobianchi,S.,&Neurodegenerativas,E.(2015).Earlyincreasing-intensitytreadmillexercisereducesneuropathicpainbypreventingnociceptorcollateralsproutinganddisruptionofchloridecotransportershomeostasisafterperipheralnerveinjury.Pain.Jun18.PMID:26090759
SilayevaL,DeebTZ,HinesM,KelleyMR,MunozMB,LeeHHC,BrandonNJ,DunlopJ,MaguireJ,DaviesPA,MossSJ.(2015)KCC2activityiscriticalinlimitingtheonsetandseverityofstatusepilepticus.PNAS.Mar2.pii:201415126.PMID:25733865
Mòdol,L.,Casas,C.,Llidó,A.,Navarro,X.,Pallarès,M.,&Darbra,S.(2014).NeonatalallopregnanoloneorfinasterideadmiNISTrationmodifieshippocampalK+Cl−co-transporterexpressionduringearlydevelopmentinmalerats.TheJournalofsteroidbiochemistryandmolecularbiology,143,343-347.PMID:24861264
Modol,L.,Cobianchi,S.,&Navarro,X.(2014).PreventionofNKCC1phosphorylationavoidsdownregulationofKCC2incentralsensorypathwaysandreducesneuropathicpainafterperipheralnerveinjury.PAIN,155(8),1577-1590.PMID:24813295
Mòdol,L.,Mancuso,R.,Alé,A.,Francos-Quijorna,I.,&Navarro,X.(2014).DifferentialeffectsonKCC2expressionandspasticityofALSandtraumaticinjuriestomotoneurons.Frontiersincellularneuroscience,8:7.PMID:24478630
LeeHH,DeebTZ,WalkerJA,DaviesPA,MossSJ(2011)NMDAreceptoractivitydownregulatesKCC2resultingindepolarizingGABAAreceptor-mediatedcurrents.NatNeurosci.Jun;14(6):736-43.PMID:21532577
SarkarJ,WakefieldS,MacKenzieG,MossSJandMaguireJ.(2011)Neurosteroidogenesisisrequiredforthephysiologicalresponsetostress:roleofneurosteroid-sensitiveGABAAreceptors.JNeurosci.31(50):18198-210.PMID:22171026
