Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Bovine,Human,Mouse,Non-humanPrimate,Pig,Rat,Sheep
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~135kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1551-1007,RRID:AB_2716769
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr1007ofmouseKCC2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-andnon-phosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
KCC2iswidelythoughttobeexpressedexclusivelyinneuronswhereitisresponsIBLeformaintaininglowintracellularchlorideconcentrationtodrivehyperpolarizingpost-synapticresponsestotheinhibitoryneurotransmittersGABAandglycine(Leeetal.,2007).KCC2isexpressedinmostadultneurons,andexpressionlevelscorrelatewellwiththematurationstateofneurons.N-ethylmaleimide(NEM)hasbeenshowtoincreasephosphorylationoftheSer940residue,whiledecreasingphosphorylationofT1007residue.DephosphorylationofresiduesT906andT1007correlateswithincreasedKCC2activity(Mossetal.,2017).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Conway,L.C.,Cardarelli,R.A.,Moore,Y.E.,Jones,K.,McWilliams,L.J.,Baker,D.J.,Burnham,M.P.,Bürli,R.W.,Wang,Q.,Brandon,N.J.andMoss,S.J.,2017.N-ethylmaleimideincreasesKCC2activitybymodulatingtransporterphosphorylation.JournalofBiologicalChemistry,pp.jbc-M117.
