Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:500
- Species:
- Rabbit
- MolecularReference:
- ~48kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p196-28,RRID:AB_2492197
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser28ofp62.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
p62,alsoknownassequestosome1(SQSTM1),isashuttleproteintransportingpolyubiquitinatedproteinsforbothproteasomalandlysosomaldegradation.p62isanintegralcomponentofinclusionsinbrainsofvariousneurodegenerativedisorders,includingAlzheimerdisease(AD)neurofibrillarytangles(NFTs)andLewybodiesinParkinsondisease(Nogalaskaetal.,2009).p62playsanimportantroleintheprotectionofcellsfromthetoxicityofmisfoldedproteinsbyenhancingaggregateformationespeciallyinthelaterstages(Nakasoetal.,2004).PhosphorylationofSer28hasrecentlybeendemonstratedtoberelatedtothepathogenesisofParkinson’sdisease.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
NogalskaA,TerraccianoC,D’AgostinoC,EngelWK,AskanasV(2009).p62/SQSTM1isoverexpressedandprominentlyaccumulatedininclusionsofsporADIcinclusion-bodymyositismusclefibers,andcanhelpdifferentiatingitfrompolymyositisanddermatomyositis.ActaNeuropathol.3:407-13.
NakasoK,YoshimotoY,NakanoT,TakeshimaT,FukuharaY,YasuiK,AragaS,YanagawaT,IshiiT,NakashimaK.(2004).Transcriptionalactivationofp62/A170/ZIPduringtheformationoftheaggregates:possIBLemechanismsandtheroleinLewybodyformationinParkinson’sdisease.BrainRes.1-2:42-51.
