Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~62kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p196-269,RRID:AB_2492198
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr269/Ser272ofhumanp62.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Theproteinscaffoldandsignalingregulatorp62(alsoknownassequestosome1(SQSTM1))isimportantincriticalcellularfunctions,includingbonehomeostasis,obesity,andcancer,becauseofitsinteractionswithvarioussignalingintermediaries.p62isoverexpressedinmanyhumancancersandisinducedduringcelltransformation.cdk1phosphorylatesp62invitroandinvivoatThr<sup>269</sup>andSer<sup>272</sup>,whichisnecessaryforthemaintenanceofappropriatecyclinB1levelsandthelevelsofcdk1activitynecessarytoallowcellstoproperlyenterandexitmitosis(Moscatetal.,2011).Thelackofcdk1-mediatedphosphorylationofp62leadstoafasterexitfrommitosis,translatingintoenhancedcellproliferationandtumOrigenesisinresponsetoRas-inducedtransformation(Moscatetal.,2011).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
MoscatJ,LinaresJF,AmanchyR,Diaz-MecoMT(2011)Phosphorylationofp62bycdk1ControlstheTimelyTransitofCellsthroughMitosisandTumorCellProliferation.MolCellBiol.1:105-17.
PankivS,LamarkT,BruunJA,ØvervatnA,BjørkøyG,andJohansen(2010).NucleocytoplasmicShuttlingofp62/SQSTM1andItsRoleinRecruitmentofNuclearPolyubiquitinatedProteinstoPromyelocyticLeukemiaBodies.JBiolChem.8:5941-53
