Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~68kDato~70kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p187-402,RRID:AB_2492200
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr402ofratp21activatedkinase2(PAK-2).ThepeptidesequenceusedisidenticalinPAK-1,2and3.Note:Thr402inPAK-2correspondstoThr423inhumanPAK-1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Inmammals,thereareseveralidentifiedisoformsofp21-activatedproteinkinasesorPAKs:α-PAK(alsoknownasPAK-1)andβ-PAK(alsoknownasPAK-3)aremostlybrain-specific,while-PAK(alsoknownasPAK-2)isexpressedubiquitously(Jakobietal.,2003).MutationsofthegenecodingforPAK-3areassociatedwithX-linkedmentalretardationandrecentworkindicatesthatPAK-3isakeyregulatorofsynapseformationandplasticityinthehippocampus(Bodaetal.,2004).PAK-3isthoughttoplayakeyroleinregulationofcellshapeandmotilityaswellascelldeath(Jakobietal.,2003;Walteretal.,1998).AutophosphorylationofThr402intheproteinhasbeenfoundtobeessentialforactivationofPAK(Jakobietal.,2000).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Nie,J.,Lilley,B.N.,Pan,Y.A.,Faruque,O.,Liu,X.,Zhang,W.,Sanes,J.R.,Han,X.andShi,Y.,2013.SAD-Apotentiatesglucose-stimulatedinsulinsecretionasamediatorofglucagon-likepeptide1responseinpancreaticβcells.Molecularandcellularbiology,33(13),pp.2527-2534. PMID:23629625
JiaNie,ChaoSun,OmarFaruque,GuangmingYe,JiaLi,QiangrongLiang,ZhijieChang,WannianYang,XiaoHan,andYuguangShi(2012)SynapsesofAmphidsDefective(SAD-A)KinasePromotesGlucose-stimulatedInsulinSecretionthroughActivationofp21-activatedKinase(PAK1)inPancreaticβ-Cells.J.Biol.Chem.287:26435-26444.PMID:22669945
