Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1,000
- Species:
- Mouse
- MolecularReference:
- ~62kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#319-COBRA,RRID:AB_2492059
Antigen/Purification:Collapse
TheantigenisarecombinantfulllengthhumanCOBRA1purifiedfromE.coli.
TheantibodyisproteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
COBRA1(cofactorofBRCA1)alsoknownasNELF-B,isanessentialcomponentofthenegativeelongationfactor(NELF)complex.COBRA1hasbeenshowntodirectlybindestrogenreceptoralpha(ERα)andrepressERα-mediatedtranscription(AiyarSEetal.,2004).COBRA1hasalsobeendemonstratedtointeractwithc-Junandc-FossuggestingaroleinmodulationoftheAP-1pathwayandthereforemaybeimportantincellproliferation,differentiation,apoptosisandoncogenesis(ZhongHetal.,2004)
Storage
100µlinPBS+0.05%NaN3.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
Aiyar,SE,SunJ,BlairAL,MoskalukCA,LuY,YeQ,YamaguchiY,MukherjeeA,RenD,HandaH,LiR(2004).Attenuationofestrogenreceptoralpha-mediatedtranscriptionthroughestrogen-stimulatedrecruitmentofanegativeelongationfactor.GenesDev.18(17):2134–46.
Zhong,H,ZhuJ,ZhangH,DingL,SunY,HuangC,YeQi(2004).COBRA1inhibitsAP-1transcriptionalactivityintransfectedcells.Biochem.Biophys.Res.Commun.325(2):568–73.
