Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Non-humanprimate,Sheep
- Applications:
- WB1:500
- Species:
- Rabbit
- MolecularReference:
- ~142kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1030-554,RRID:AB_2560942
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser554ofratDENND3.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
TheDENN(differentiallyexpressedinnormalandneoplasticcells)domain(DENND)isapoorlycharacterizedproteinmoduleconservedthroughoutevolution(McPhersonetal.,2011).ProteinsbearingaDENNdomainhaverecentlyemergedasthelargestfamilyofRabGEFs.AmongtheseDENNdomainproteins,DENND3isaGEFforRab12;promotingtheexchangeofGDPtoGTP,convertinginactiveGDP-boundRAB12intoitsactiveGTP-boundform(McPhersonandXu,2015;Matsuietal.,2011).ULK-mediatedphosphorylationofDENND3atserines554and572upregulatesitsGEFactivitytowardthesmallGTPaseRab12(McPhersonandXu,2015).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
McPhersonPS,XuJ(2015).DENND3:asignaling/traffickinginterfaceinautophagy.CellCycle.17:2717-8.
MatsuiT,ItohT,FukudaM(2011).SmallGTPaseRab12RegulatesConstitutiveDegradationofTransferrinReceptor.Traffic.10:1432-43.
McPhersonPS,DokainishH,MaratAL(2011).DENNDomainProteins:RegulatorsofRabGTPases.JBiolChem.16:13791-800.
