>
产品中心 >
Functional_antibody >
PhosphoSolutions/DsbA-L (Disulfide-bond-A oxidoreductase-like protein) Antibody/465-DsbA-L/100 µl
Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- Serum
- Specificity:
- Mouse,Human
- Applications:
- WB1:2,000IHC1:200
- Species:
- Rabbit
- MolecularReference:
- ~25kDa
- CiteThisAntibody:
- (PhosphoSolutionsCat#465-DsbA-L,RRID:AB_2492080)
Antigen/Purification:Collapse
TheantigenisafulllengthrecombinantmouseDsbA-Lprotein.
Theantibodyisserum.
BIOLOGicalSignificance:Collapse
Disulfide-bond-Aoxidoreductase-likeprotein(DsbA-L,previouslynamedasGSTKappa)isanADIponectin-interactingprotein.DsbA-Lishighlyexpressedinadiposetissue,anditsexpressionlevelisnegativelycorrelatedwithobesityinmiceandhumans.DsbA-Lexpressionin3T3-L1adipocytesisstimulatedbytheinsulinsensitizerrosiglitazoneandinhibitedbytheinflammatorycytokineTNFalpha.PolymorphismofDsbA-Lgenehasrecentlybeenimplicatedininsulinsecretionandbodyfatdistribution(GaoFetal.,2009).OverexpressionofDsbA-LpromotesadiponectinmultimerizationwhilesuppressingDsbA-LexpressionbyRNAimarkedlyandselectivelyreducesadiponectinlevelsandsecretionin3T3-L1adipocytes.RecentstudiesidentifyDsbA-Lasakeyregulatorforadiponectinbiosynthesis(Liuetal.,2008).
Storage
Liquidin40%glycerol.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1yearafterdateofreceipt.Avoidfreeze/thawcycles.
ProductSpecificReferences
DeClercq,V.,d’Eon,B.,&McLeod,R.S.(2015).Fattyacidsincreaseadiponectinsecretionthroughbothclassicalandexosomepathways.BiochimicaetBiophysicaActa(BBA)-MolecularandCellBiologyofLipids.Sep;1851(9):1123-33.PMID:25900100
WangA,LiuM,LiuX,DongLQ,GlickmanRD,SlagaTJ,ZhouZ,LiuF(2011)Up-regulationofAdiponectinbyResveratrol:TheEssentialRolesofAkt/FOXO1andAMP-activatedproteinKinaseSignalingPathwaysandDsbA-L.JBiolChem.Jan7;286(1):60-6.PMID:20980258
Liuetal,(2008)Adisulfide-bondAoxidoreductase-likeprotein(DsbA-L)regulatesadiponectinmultimerization.ProcNatlAcadSciUSA,105,18302-18307.PMID:19011089
