Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000IHC1:500
- Species:
- Rabbit
- GeneName:
- MAPK1/2
- MolecularReference:
- ~42-44kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p160-2024,RRID:AB_2492090
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr202andTyr204ofratERK/MAPK.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Extracellular-SignalRegulatedKinase/Mitogen-ActivatedProteinKinase(ERK/MAPK)isanintegralcomponentofcellularsignalingduringmitogenesisanddifferentiationofmitoticcellsandalsoisthoughttoplayakeyroleinlearningandmemory(AdamsandSweatt,2002;Ahn,1993;TanoueandNishida,2003;JohnsonandLapadat,2002).TheactivityofthiskinaseisregulatedbydualphosphorylationatThr202andTyr204(Ahn,1993).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Erickson,C.A.,Ray,B.,Wink,L.K.,Bayon,B.L.,Pedapati,E.V.,Shaffer,R.,Schaefer,T.L.andLahiri,D.K.,2016.Initialanalysisofperipherallymphocyticextracellularsignalrelatedkinaseactivationinautism.JournalofPsychiatricResearch.
Morgan,J.E.,Shanderson,R.L.,Boyd,N.H.,Cacan,E.,&Greer,S.F.(2015).TheclassIItransactivator(CIITA)isregulatedbypost-translationalmodificationcross-talkbetweenERK1/2phosphorylation,mono-ubiquitinationandLys63ubiquitination.Biosciencereports,35(4),e00233.PMID:26181363
