Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- MAPK1/2
- MolecularReference:
- ~42kDa-44kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#500-ERK,RRID:AB_2492089
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminusofratERK/MAPK.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurification.
BIOLOGicalSignificance:Collapse
Extracellular-SignalRegulatedKinase/Mitogen-ActivatedProteinKinase(ERK/MAPK)isanintegralcomponentofcellularsignalingduringmitogenesisanddifferentiationofmitoticcellsandalsoisthoughttoplayakeyroleinlearningandmemory(AdamsandSweatt,2002;Ahn,1993;TanoueandNishida,2003;JohnsonandLapadat,2002).TheactivityofthiskinaseisregulatedbydualphosphorylationatThr202andTyr204(Ahn,1993).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Erickson,C.A.,Ray,B.,Wink,L.K.,Bayon,B.L.,Pedapati,E.V.,Shaffer,R.,Schaefer,T.L.andLahiri,D.K.,2016.Initialanalysisofperipherallymphocyticextracellularsignalrelatedkinaseactivationinautism.JournalofPsychiatricResearch.PMID:27743527
