Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Non-humanprimate,Porcine,Canine
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~95kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p168-1160,RRID:AB_2492137
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr1160ofhumaninsulinreceptor.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Theinsulinreceptor(IR)isawell-studiedreceptortyrosinekinasecomposedoftwoαsubunits,responsIBLefortheextracellularinsulinbindingsite,andtwoβsubunits,responsibleforintracellularproteinkinaseactivity(Endemannetal.,1990,Chiuetal.,2010).Thebindingofinsulintotheαsubunitsactivatestheintrinsickinaseactivitylocatedintheβsubunitsandsubsequentlyinitiatesacascadeofphosphorylationeventscausingmajorconformationalchangesintheactivationloopofthekinasedomain,whichleadtodifferentbiologicalfunctions(Chiuetal.,2010).IthasbeenhypothesizedthatT1160phosphorylationaffectsorisaffectedbyY1158/62/63phosphorylationandthattheconformationofT1160andpT1160islikelytobeaffectedbythephosphorylationstatusofthesurroundingtyrosines.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
ChiuSLandClineHL(2010).Insulinreceptorsignalinginthedevelopmentofneuronalstructureandfunction.NeuralDev.5:7
EndemannG,YonezawaK,andRothRA(1990).Phosphatidylinositolkinaseoranassociatedproteinisasubstratefortheinsulinreceptortyrosinekinase.J.Biol.Chem.265:396-400
