Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~45kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p180-292,RRID:AB_2492147
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr292ofhumanMEK1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
MEK1(MAPkinasekinase,alsoknownasMKK)isanintegralcomponentoftheMAPkinasecascadethatregulatescellgrowthanddifferentiation(Ahn,1993;Chongetal.,2003).Thispathwayalsoplaysakeyroleinsynapticplasticityinthebrain(AdamsandSweatt,2002).ActivatedMEK1actsasadualspecificitykinasephosphorylatingbothathreonineandatyrosineresidueonMAPkinase(Kyriakisetal.,1991;Segeretal.,1991;Crewsetal.,1992).Conversely,therealsoappearstobeafeedbackphosphorylationofMEK1byMAPkinase.ThesitesonMEK1thatarephosphorylatedbyMAPkinaseareThr292andThr386(Mansouretal.,1994).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Tassin,T.C.,Benavides,D.R.,Plattner,F.,Nishi,A.,&Bibb,J.A.(2015).RegulationofERKKinasebyMEK1KinaseInhibitionintheBrain.JournalofBiologicalChemistry,Jun26;290(26):16319-29.PMID:25971971
