Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~45kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p180-386,RRID:AB_2492148
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr386ofhumanMEK1.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
MEK1(MAPKinaseKinase,alsoknownasMKK)isanintegralcomponentoftheMAPkinasecascadethatregulatescellgrowthanddifferentiation(Ahn,1993;Chongetal.,2003).Thispathwayalsoplaysakeyroleinsynapticplasticityinthebrain(AdamsandSweatt,2002).ActivatedMEK1actsasadualspecificitykinasephosphorylatingbothathreonineandatyrosineresidueonMAPkinase(Kyriakisetal.,1991;Segeretal.,1991;Crewsetal.,1992).Conversely,therealsoappearstobeafeedbackphosphorylationofMEK1byMAPkinase.ThesitesonMEK1thatarephosphorylatedbyMAPkinaseareThr292andThr386(Mansouretal.,1994).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
AdamsJP,SweattJD(2002)Molecularpsychology:Roles fortheERKMAPkinasecascadeinmemory.AnnuRev PharmacolToxicol42:135-163.
AhnNG(1993)TheMAPkinasecascade.Discoveryofanewsignaltransductionpathway.MolCellBiochem127-128:201-209.
ChongH,VikisHG,GuanKL(2003)MechanismsofregulatingtheRafkinasefamily.CellularSignalling15:463-469.
CrewsCM,AlessandriniA,EriksonRL(1992)TheprimarystructureofMEK,aproteinkinasethatphosphorylatestheERKgeneproduct.Science258:478-480.
KyriakisJM,BrautiganDL,IngebritsenTS,AvruchJ(1991)pp54Microtubule-associatedprotein-2kinaserequiresbothtyrosineandserine/threoninephosphorylationforactivity.JBiolChem266:10043-10046.
MansourSJ,ResingKA,CandiJM,HermannAS,GloorJW,HerskindKR,WartmannM,DavisRJ,AhnNG
(1994)Mitogen-activatedprotein(MAP)kinasephosphorylationofMAPkinasekinase:Determinationofphosphorylationsitesbymassspectrometryandsite-directedmutagenesis.JBiochem(Tokyo)116:304-314.
SegerR,AhnNG,BoultonTG,YancopoulosGD,PanayotatosN,RadziejewskaE,EricssonL,BratlienRL,CobbMH,KrebsEG(1991)Microtubule-associatedprotein2kinases,ERK1andERK2,undergoautophos-phorylationonbothtyrosineandthreonineresidues:Implicationsfortheirmechanismofactivation.ProcNatlAcadSciUSA88:6142-6146.
