Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~45kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p180-21822,RRID:AB_2492146
Antigen/Purification:Collapse
Theantigenisa Phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser218,222ofhumanMEK1/2.Note:ThephosphositesinhumanMEK1/2areSer218andSer222;thephosphositesinmouseMEK1/2areSer217andSer221.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
MEK1(MAPKinaseKinase,alsoknownasMKK)isanintegralcomponentoftheMAPkinasecascadethatregulatescellgrowthanddifferentiation(Ahn,1993;Chongetal.,2003).Thispathwayalsoplaysakeyroleinsynapticplasticityinthebrain(AdamsandSweatt,2002).ActivatedMEK1actsasadualspecificitykinasephosphorylatingbothathreonineandatyrosineresidueonMAPkinase(Kyriakisetal.,1991;Segeretal.,1991;Crewsetal.,1992).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
TaoJ,HuK,ChangQ,WuH,ShermanNE,MartinowichK,KloseRJ,SchanenC,JaenischR,WangW,SunYE(2009)PhosphorylationofMeCP2atSerine80regulatesitschromatinassociationandneurologicalfunction.ProcNatlAcadSciUSA.106(12):4882-7.
