Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Human,Non-humanprimate
- Applications:
- WB1:500
- Species:
- Rabbit
- MolecularReference:
- ~52kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1180-Mnk2a,RRID:AB_2631038
Antigen/Purification:Collapse
TheantigenisapeptidefromtheC-terminalregion,specifictothehumanMnk2aisoform. ThesequenceoftheantigenhasnohomologywithMnk1orMnk2b.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
BIOLOGicalSignificance:Collapse
MAPkinase-interactingkinasesMnk1andMnk2areactivatedbytheMAPkinases,ERKandp38(Waskiewiczetal.,1997). Additionally,Mnk1andMnk2havebeenshowntophosphorylatethetranslationinitiationfactoreIF4E(FukunagaandHunter1997;Waskiewiczetal.,1997). AlternativesplicingoftheMKNK2generesultsintwoisoformswithdifferentC-termini:onethathasaMAPK-bindingdomain,Mnk2a,andonethatdoesnot,Mnk2b(Parraetal.,2005). RecentstudieshaveindicatedthatMnk2ahastumorsuppressiveactivitywhileMnk2bispro-oncogenic(Maimonetal.,2014).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
Fukunaga,R.,&Hunter,T.(1997)ANewMAPKinase-ActivatedProteinKinaseIsolatedbyaNovelExpressionScreeningMethodtoIdentifyProteinKinaseSubstrates.EMBOJ.Apr15;16(8):1921-33.
Maimon A,MogilevskyM,ShiloA,Golan-GerstlR,ObiedatA,Ben-HurV,Lebenthal-LoingerI,SteinI,ReichR,BeenstockJ,ZehoraiE,AndersenCL,ThorsenK,OrntoftTF,DavisRJ,DavidsonB,MuD,KarniR(2014)Mnk2AlternativeSplicingModulatesthep38-MAPKPathwayandImpactsRas-InducedTransformation.CellReports7,501-513.
ParraJL,BuxadéM,ProudCG(2005)FeaturesoftheCatalyticDomainsandCTerminioftheMAPKSignal-integratingKinasesMnk1andMnk2DetermineTheirDifferingActivitiesandRegulatoryProperties.J.Biol.Chem.280,37623-37633.
WaskiewiczAJ,FlynnA,ProudCG,CooperJA(1997)Mitogen-activatedProteinKinasesActivatetheSerine/ThreonineKinasesMnk1andMnk2.EMBOJ.16,1909-1920.
