Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Drosophila
- Applications:
- WB1:500
- Species:
- Rabbit
- MolecularReference:
- ~60kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p204-356,RRID:AB_2492214
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr356ofPRAS40.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
PRAS40(proline-richAkt/PKBsubstrateof40kDa)actsattheintersectionoftheAkt-andmTOR-mediatedsignalingpathwaysanditsphosphorylationbyAktandbymTORC1resultsindissociationofPRAS40frommTORC1whichmayrelieveaninhibitoryconstraintonmTORC1activity(Wizaetal.,2012).PhosphorylationofPRAS40byAktandassociationwith14-3-3hasalsobeenindicatedtobecrucialforinsulintostimulatemTOR.(VanderHaaretal.,2007).TheprimaryfunctionofPRAS40invivoinDrosophilahasbeenshowntoregulateTORC1activity,andnottoactasadownstreamtargetandeffectorofTORC1(Pallaresetal.,2012).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
WizaC,NascimentoEBM,andOuwensDM(2012).RoleofPRAS40inAktandmTORsignalinginhealthanddisease.AJPENDO.302:E1453–E1460.
VanderHaarE,LeeSI,BandhakaviS,GriffinTJ,KimDH.(2007).InsulinsignalingtomTORmediatedbytheAkt/PKBsubstratePRAS40.NatCellBiol3:316-23
PallaresC,Cakan-AkdoganG,andTelemanAA(2012).Tissue–SpecificCouplingbetweenInsulin/IGFandTORC1SignalingviaPRAS40inDrosophila.DevelopmentalCell.22:172-8
