Rabbitpolyclonalantibody
- Size:
- 100µl
- Formulation:
- AffinityPurified
- Specificity:
- Rat,Mouse,Human,Xenopus,Zebrafish
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~120kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p200-48260,RRID:AB_2492210
Antigen/Purification:Collapse
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer482,486,490ofXenopuspolo-likekinasekinase(PLKK).
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Considerableevidenceindicatesthatapolo-likekinase(PLK)playsanimportantroleincellcycleregulation.PLKisalsorequiredforbipolarspindleformation,activationoftheanaphase-promotingcomplex/cyclosome,andcytokinesis.RecentworkledtotheidentificationofaPLKKthatisthoughttoberesponsIBLeforactivationofPLK.Recentwork(Erikson,etal.,2004)hasshownthatPLKKisinturnactivatedbyphosphorylationatthreesites(Ser482,Ser486andSer490).ThusactivationofPLKisthoughttoinvolveakinasecascadeinvolvingthephosphorylationofSer482,486,490inPLKK
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
EriksonE,HaysteadTAJ,Qian,Y-W,MallerJL(2004)Afeedbackloopinthepolo-likekinaseactivationpathway.JBiolChem279:32219-32224.
KumagaiA,DunphyWG(1996)PurificationandmolecularcloningofPlx1,acdc25-regulatorykinasefromXenopuseggextracts.Science273:1377-1380.LiuJ,LewellynAL,ChenLG,MallerJL(2004)ThepoloboxisrequiredformultiplefunctionsofPlx1inmitosis.JBiolChem279:21367-21373.
vanVugt,MATM,vandeWeerdt,BCM,VaderG,JanssenH,CalafatJ,KlompmakerR,WolthuisRMF,MedemaRH(2004)Polo-likekinase-1isrequiredforbipolarspindleformationbutisdispensableforanaphasepromotingcomplex/cdc20activationandinitiationofcytokinesis.JBiolChem279:36841-36854.
EleanorErikson,TimothyA.J.Haystead,Yue-WeiQian,andJamesL.Maller(2004)AFeedbackLoopinthePolo-likeKinaseActivationPathway.J.Biol.Chem.,Jul2004;279:32219–32224
