Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Chicken
- Applications:
- IHC1:1,000
- Species:
- Mouse
- CiteThisAntibody:
- PhosphoSolutionsCat#1685-PROX1,RRID:AB_2492217
Antigen/Purification:Collapse
TheantigenisarecombinanthumanProx1protein.
TheantibodyisaProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
Prox1(Prospero-relatedhomeobox1)isadivergenthomeogenethatregulatescellproliferation,fatedeterminationanddifferentiationduringvertebrateembryonicdevelopment.Theproteinisexpressedinthedevelopinglensandisalsodetectedinembryonicbrain,lung,liverandkidney;whileinadultitismoreabundantinheartandliverthaninbrain,skeletalmuscle,kidneyandpancreas(Chenetal.,2006).Prox1isthoughttoplayafundamentalroleinearlydevelopmentoftheCNSandmayregulategeneexpressionanddevelopmentofpostmitoticundifferentiatedyoungneurons(Misraetal.,2008)
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
GeneralReferences
XiaorenChen,TapanP.Patel,WilliamJ.Cain,MelindaK.Duncan.ProductionofmonoclonalantibodiesagainstProx1.Hybridoma(2006)Feb;25(1):27-33.
MisraK,GuiH,MatiseMP(2008)Prox1regulatesatransitorystateforinterneuronneurogenesisinthespinalcord.DevDyn237(2):393-402.
