Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Chicken,Non-humanprimate,Zebrafish,GuineaPig
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~28kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1860-244,RRID:AB_2492234
Antigen/Purification:Collapse
Theantigenis aphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser244ofmouserpS6.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
RibosomalproteinS6(rpS6)isacriticalcomponentofthe40Sribosomalsubunitthatmediatestranslationinitiationatthe5′-m7GpppGcapofmRNA.TherpS6proteinisbothcytoplasmicandnuclearlocalized(ChenandDittmer2011).Inresponsetomitogenicstimuli,rpS6undergoesorderedC-terminalphosphorylationbyp70S6kinasesandp90ribosomalS6kinasesonfourSerresidues(Ser235,Ser236,Ser240,andSer244)whosemodificationpotentiatesrpS6capbindingactivity(Hutchinsonetal.,2011).Additionally,rpS6phosphorylationandfunctionarehighlyregulatedandhavebeenimplicatedintheregulationoftranslationalinitiationandproteinsynthesisinresponsetoextracellularstimulisuchasTRAILandgammainterferon(IFN-γ),aswellasuponactivationofthephosphatidylinositol3-kinase(PI3K)–Akt–mTORpathway(ChenandDittmer2011).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
ChenWandDittmerD(2011)RibosomalproteinS6(RPS6)interactswiththelatency-associatednuclearantigen(LANA)ofKaposisarcomaassociatedherpesvirus(KSHV).J.Virol.189495-9505
FumagalliS.andThomas,G.(2000).S6phosphorylationandsignaltransduction.InTranslationalcontrolofgeneexpression(eds.N.Sonenbergetal.),pp.695-717.Cold
SpringHarborLaboratoryPress,ColdSpringHarbor,NY.HutchinsonJA,ShanwareNP,ChangH,TibbettsRS.(2011)RegulationofribosomalproteinS6phosphorylationbycaseinkinase1andproteinphosphatase1.JBiolChem10:8688-96
