Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~59,65,68kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1573-416,RRID:AB_2492257
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser416ofrattau.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Tauisakeymicrotubule-associatedproteinthatplaysanimportantroleintheformationofmicrotubulesinaxons(Binderetal.1985).SixtauisoformshavebeenidentifiedasproductsofasinglegeneproducedbyalternativemRNAsplicing(Goedert1990).TaumutationshavebeenimplicatedinmanyneurodegenerativedisorderssuchasAlzheimer’sdisease(AD),Pick’sdiseaseandprogressivesupranuclearpalsy.IthasbeenwelldocumentedthathyperphosphorylatedtauisamajorcomponentofpairedhelicalfilamentsinADbrain(Lee1995).Serine416hasbeendemonstratedtobeamajorphosphorylationsiteinvitrobyCaMkinaseII(Steineratal.1990).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
BinderLI,FrankfurterA,RebhunLI(1985)Thedistributionoftauinthemammaliancentralnervoussystem.JCellBioOct;101(4):1371-8.
LeeV.M.Y.(1995)DisruptionoftheCytoskeletoninAlzheimer’sdisease.Curr.Opin.Neurobiol.5,663-668.
GoedertM.andJakesR.(1990)Expressionofseparateisoformsofhumantauprotein:correlationwiththetaupatterninbrainandeffectsontubulinpolymerization.EMBOJ9,4225-4230.
SteinerB.,MandelkowE.M.,BiernatJ.etal.(1990)Phosphorylationofmicrotubuleassociatedproteintau:identificationofthesiteforCa2+-calmodulindependentkinaseandrelationshipwithtauphosphorylationinAlzheimertangles.EMBOJ.9,3539-3544.
