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Functional_antibody >
PhosphoSolutions/Thyroid Hormone Receptor Antibody, α1/α2-Isotype/1220-TRa/100 µl
Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Mouse
- MolecularReference:
- ~50kDaand~58kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1220-TRa,RRID:AB_2492261
Antigen/Purification:Collapse
TheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionofhumanthyroidhormonereceptor,α1/α2-isotype.
TheantibodyispreparedfrommouseascitesbyammoniumsulfateprecipitationfollowedbyaffinitypurificationonaProteinGcolumn.
BIOLOGicalSignificance:Collapse
Thyroidhormonesareessentialfordevelopmentofthecentralnervoussystemanddeficitsinthesehormonesduringdevelopmentaffectssuchcognitivefunctionsaslearningandmemory(Ambroginietal.,2005;ChanandKilby,2000).ThyroidhormonesexerttheirphysiologicalrolemainlythroughbindingtospecificnuclearreceptorsincludingthepredominantisoformsofthyroidhormonereceptorsTRα1,TRα2,TRβ1andTRβ2.TRα1,TRβ1andTRβ2bindT3withhighaffinityandalsobindtothyroidhormoneresponseelements(TREs)onchromatintoregulatethetranscriptionalprocessesinseveraltargettissues,includingadultratbrain(Constantinouetal.,2005).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
AmbroginiP,CuppiniR,FerriP,ManciniC,CiaroniS,VociA,GerdoniE,GalloG(2005)Thyroidhormonesaffectneurogenesisinthedentategyrusofadultrat.Neuroendocrinology81:244-253.
ChanS,KilbyMD(2000)Thyroidhormoneandcentralnervoussystemdevelopment.JEndocrinol165:1-8.
ConstantinouC,MargarityM,ValcanaT(2005)Region-specificeffectsofhypothyroidismontherelativeexpressionofthyroidhormonereceptorsinadultratbrain.MolCellBiochem278:93-100.
