Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000IHC1:500
- Species:
- Rabbit
- GeneName:
- SYN1
- MolecularReference:
- ~78kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1560-549,RRID:AB_2492246
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser549ofratsynapsinI.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
SynapsinIplaysakeyroleinsynapticplasticityinbrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Theroleofsynapsininsynapticplasticityandinsynaptogenesisisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).Ser549alongwithSer62andSer67arethesitesofsynapsinIthatarephosphorylatedbyMAPkinase(Jovanovicetal.,1996).Phosphorylationandsubsequentdephosphorylationofthissiteisthoughttoplayakeyroleinsynapticvesicletrafficking.
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
JovanovicJN,Benfenati,F,SiowYL,SihraTS,SangheraJS,PelechSL,GreengardP,CzernikAJ(1996)NeurotrophinsstimulatephosphorylationofSynapsinIbyMAPkinaseandregulateSynapsinI-actininteractions.Neurobiology93:3679-3683.
CzernikAJ,PangDT,GreengardP(1987)AminoacidsequencessurroundingthecAMP-dependentandcalcium/calmodulin-dependentphosphorylationsitesinratandbovinesynapsinI.ProcNatlAcadSci(USA)84:7518-7522.
FengJ,ChiP,BlanpiedTA,XuYM,MagarinosAM,FerreiraA,TakahashiRH,KaoHT,McEwenBS,RyanTA,AugustineGJ,GreengardP(2002)RegulationofneurotransmitterreleasebysynapsinIII.JNeurosci22:4372-4380.
JovanovicJN,SihraTS,NairnAC,HemmingsHC,Jr.,GreengardP,CzernikAJ(2001)OpposingchangesinphosphorylationofspecificsitesinsynapsinIduringCa2+-dependentglutamatereleaseinisolatednerveterminals.JNeurosci21:7944-7953.
KaoHT,SongHJ,PortonB,MingGL,HohJ,AbrahamM,CzernikAJ,PieriboneVA,PooMM,GreengardP(2002)AproteinkinaseA-dependentmolecularswitchinsynapsinsregulatesneuriteoutgrowth.NatureNeurosci5:431-437.
NayakAS,MooreCI,BrowningMD(1996)CAMkinaseIIphosphorylationofthepresynapticproteinsynapsinispersistentlyincreasedduringexpressionoflong-termpotentiation.ProcNatlAcadSci(USA)93:15451-15456.
