Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Zebrafish
- Applications:
- WB1:1000
- Species:
- Rabbit
- GeneName:
- SYN1
- MolecularReference:
- ~78kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1560-603,RRID:AB_2492247
Antigen/Purification:Collapse
Theantigenis Phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser603ofsynapsinI.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
SynapsinIplaysakeyroleinsynapticplasticityinbrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Theroleofsynapsininsynapticplasticityandinsynaptogenesisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).Serine603isthesiteonsynapsinIthatisphosphorylatedbycalciumcalmodulinkinaseIIandbyp21-activatedkinases(Sakuradaetal.,2002;Czerniketal.,1987).Phosphorylationofthissiteisthoughttoregulatesynapticvesiclefunction(Nayaketal.,1996;BahlerandGreengard,1987;McGuinnessetal.,1989).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Magupalli,V.G.,Mochida,S.,Yan,J.,Jiang,X.,Westenbroek,R.E.,Nairn,A.C.,Scheuer,T.&Catterall,W.A.(2013).Ca2+-independentactivationofCa2+/calmodulin-dependentproteinkinaseIIboundtotheC-terminaldomainofCaV2.1calciumchannels.JournalofBiologicalChemistry,288(7),4637-4648.PMID:23255606
SergioLeal-Ortiz,ClarissaL.Waites,RyanTerry-Lorenzo,PedroZamorano,EckartD.Gundelfinger,andCraigC.Garner(2008)PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.J.CellBiol.,181:831–846.PMID:18519737
