Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Xenopus,Bovine,Zebrafish
- Applications:
- WB1:1000IHC1:500
- Species:
- Rabbit
- GeneName:
- SYN1
- MolecularReference:
- ~78kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1560-9,RRID:AB_2492244
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser9ofsynapsinI.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
SynapsinIplaysakeyroleinsynapticplasticityinbrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Inadditiontoitsroleinplasticity,theexpressionofsynapsinIisapreciseindicatorofsynapseformation(MooreandBernstein,1989;Stoneetal.,1994).Thus,synapsinIimmunocytochemistryprovidesavaluabletoolforthestudyofsynaptogenesis.Theroleofsynapsininsynapticplasticityandinsynaptogenesisisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).Serine9isthesiteonsynapsinIthatisphosphorylatedbycAMP-dependentproteinkinaseandbycalciumcalmodulinkinaseI(Czerniketal.,1987).Phosphorylationofthissiteisthoughttoregulatesynapticvesiclefunctionandneuriteoutgrowth(Kaoetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Benleulmi‐Chaachoua,A.,Chen,L.,Sokolina,K.,Wong,V.,Jurisica,I.,Emerit,M.B.,Darmon,M.,Espin,A.,Stagljar,I.,Tafelmeyer,P.,Zamponi,G.W.,Delagrange,P.,Maurice,P.,&Jockers,R.(2015).ProteininteractomeminingdefinesmelatoninMT1receptorsasintegralcomponentofpresynapticproteincomplexesofneurons.JournalofPinealResearch.PMID:26514267
YoavShulman,AlexandraStavsky,TatianaFedorova,DanMikulincer,MeravAtias,IgalRADInsky,JoyKahn,InnaSlutsky,DanielGitler.(2015).ATPBindingtoSynapsinIIaRegulatedUsageandClusteringofVesiclesinTerminalsofHippocampalNeurons.J.Neuro,35(3):985-998.PMID:25609616
SachikoShimomura,TadashiNagamine,NaoyaHatano,NoriyukiSueyoshi,andIsamuKameshita(2010)Identificationofanendogenoussubstrateofzebrafishdoublecortin-likeproteinkinaseusingahighlyactivetruncationmutant.J.Biochem.,147:711–722.PMID:20097902
