Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1000IF1:1,000IHC1:1,000IP5µlper200µglysate
- Species:
- Rabbit
- GeneName:
- SYN1
- MolecularReference:
- ~78kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1926-SYNP,RRID:AB_2492242
Antigen/Purification:Collapse
Theantigenisanativeproteinpurifiedfrombovinebrain.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthenativeproteinwascoupled.
BIOLOGicalSignificance:Collapse
SynapsinIplaysakeyroleinsynapticplasticityinbrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Inadditiontoitsroleinplasticity,theexpressionofsynapsinIisapreciseindicatorofsynapseformation(MooreandBernstein,1989;Stoneetal.,1994).Thus,synapsinIimmunocytochemistryprovidesavaluabletoolforthestudyofsynaptogenesis.Theroleofsynapsininsynapticplasticityandinsynaptogenesisisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Song,S.H.,&Augustine,G.J.(2016).SynapsinisoformsregulatingGABAreleasefromhippocampalinterneurons.TheJournalofNeuroscience,36(25),6742-6757.PMID:27335405
Wahba,G.,Hebert,A.E.,Grynspan,D.,Staines,W.,&Schock,S.(2016).Arapidandefficientmethodfordissociatedculturesofmousemyentericneurons.Journalofneurosciencemethods,261,110-116.PMID:26706461
Magupalli,V.G.,Mochida,S.,Yan,J.,Jiang,X.,Westenbroek,R.E.,Nairn,A.C.,Scheuer,T.&Catterall,W.A.(2013).Ca2+-independentactivationofCa2+/calmodulin-dependentproteinkinaseIIboundtotheC-terminaldomainofCaV2.1calciumchannels.JournalofBiologicalChemistry,288(7),4637-4648. PMID:23255606
KurtisD.Davies,SusanM.Goebel-Goody,StevenJ.Coultrap,andMichaelD.Browning(2008)LongTermSynapticDepressionThatIsAssociatedwithGluR1DephosphorylationbutNot-Amino-3-hydroxy-5-methyl-4-isoxazolepropionicAcid(AMPA)ReceptorInternalizationJ.Biol.Chem.,283:33138–33146.PMID:18819923
