Rabbitpolyclonalantibody,neatserum
- Size:
- 50µl
- Formulation:
- Neatserum
- Specificity:
- Rat,Mouse,Human
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~78kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1927-SYNP,RRID:AB_2492243
Antigen/Purification:Collapse
Theantigenisanativeproteinpurifiedfrombovinebrain.
Theantibodyisunpurifiedneatserum.
BIOLOGicalSignificance:Collapse
SynapsinIplaysakeyroleinsynapticplasticityinthebrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Inadditiontoitsroleinplasticity,theexpressionofsynapsinIisapreciseindicatorofsynapseformation(MooreandBernstein,1989;Stoneetal.,1994).Thus,SynapsinIimmunocytochemistryprovidesavaluabletoolforthestudyofsynaptogenesis.Theroleofsynapsininsynapticplasticityandinsynaptogenesisisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).
Storage
50μlneatserum.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
FengJ,ChiP,BlanpiedTA,XuYM,MagarinosAM,FerreiraA,TakahashiRH,KaoHT,McEwenBS,RyanTA,AugustineGJ,GreengardP(2002)RegulationofneurotransmitterreleasebysynapsinIII.JNeurosci22:4372-4380.
JovanovicJN,SihraTS,NairnAC,HemmingsHC,Jr.,GreengardP,CzernikAJ(2001)OpposingchangesinphosphorylationofspecificsitesinsynapsinIduringCa2+-dependentglutamatereleaseinisolatednerveterminals.JNeurosci21:7944-7953.
KaoHT,SongHJ,PortonB,MingGL,HohJ,AbrahamM,CzernikAJ,PieriboneVA,PooMM,GreengardP(2002)AproteinkinaseA-dependentmolecularswitchinsynapsinsregulatesneuriteoutgrowth.NatureNeurosci5:431-437.
MooreRY,BernsteinM(1989)SynaptogenesisintheratsuprachiasmaticnucleusdemonstratedbyelectronmicroscopyandsynapsinIimmunoreactivity.JNeurosci9:2151-2162.
NayakAS,MooreCI,BrowningMD(1996)CaMkinaseIIphosphorylationofthepresynapticproteinsynapsinispersistentlyincreasedduringexpressionoflong-termpotentiation.ProcNatlAcadSci(USA)93:15451-15456.
StoneLM,BrowningMD,FingerTE(1994)Differentialdistributionofthesynapsinsintheratolfactorybulb.JNeurosci14:301-309.
Note:Dr.MichaelBrowningandDr.AndrewCzernik,co-authorsofthecitedpapersarethePresidentandChiefScientificOfficerofPhosphoSolutionsandtwoofitsfounders.
