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Functional_antibody >
PhosphoSolutions/Synaptobrevin (VAMP) Antibody/1933-SYB/100 µl
Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Mouse,Human,Bovine,Porcine,Rabbit
- Applications:
- WB1:1,000IHC1:100
- Species:
- Mouse
- MolecularReference:
- ~16kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1933-SYB,RRID:AB_2492248
Antigen/Purification:Collapse
Theantigenisasynapticimmunoprecipitate(crude)fromhumanbrain.
TheantibodyisProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
Synaptobrevin(akaVAMP)isanintegralmembraneproteinofsynapticvesiclesthatplaysamajorroleintheformationoflargerSNAREcomplexes,alongwithSNAP-25andsyntaxin.Synaptobrevinhasbeenshowntobeessentialfortwofastsynapse-specificmembranetraffickingprocesses:fastexocytosisforneurotransmitterreleaseandfastendocytosisthatmediatesrapidrecyclingofsynapticvesicles(Deaketal.,2004).DecreasedlevelsofsynaptobrevininhumanhippocampusandcortexhavebeencorrelatedwithcognitivedeficitsinAlzheimer’sdisease(Szeetal.,2000).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
DeákF,SchochS,LiuX,SüdhofTC,KavalaliET(2004)Synaptobrevinisessentialforfastsynaptic-vesicleendocytosis.NatCellBiol.2004Nov;6(11):1102-8.
SzeCI,BiH,Kleinschmidt-DeMastersBK,FilleyCM,MartinLJ(2000)SelectiveregionallossofexocytoticpresynapticvesicleproteinsinAlzheimer’sdiseasebrains.JNeurolSci.175(2):81-90.
Honer,W.G.,Hu,L.&Davies,P.Humansynapticproteinswithaheterogeneousdistributionincerebellumandvisualcortex(1993).BrainResearch609,9-20.
