Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat
- Applications:
- WB1:1,000IHC1:100
- Species:
- Mouse
- MolecularReference:
- ~145kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1936-SYNJ,RRID:AB_2492249
Antigen/Purification:Collapse
TheantigenisarecombinantproteincorrespondingtoaminoacidresiduesfromtheC-terminalregionofratsynaptojanin1.
TheantibodyisProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
Synaptojaninisaphosphatidylinositolphosphataseinvolvedinclathrin-mediatedendocytosisofsynapticvesicles.Synaptojanin1hastwoalternativelysplicedisoforms;onethatis~145kDAandisexclusivelyexpressedinneurons,andtheother~170kDawhichisexpressedinnon-neuronal,peripheraltissues(RamjaunAR&McPhersonPS,1996).ThegenewhichencodesSynaptojanin1,SYNJ1,hasbeenmappedtochromosome21thusmakingitacandidateforinvolvementinDown’ssyndrome(DS).IthasrecentlybeendemonstratedthatTs65Dnmice(themostcommonlyusedmodelofDS)havealteredphosphatidylinositol-4,5-bisphosphatemetabolism.ThisdefectisrescuedbyrestoringSYNJ1todisomyintheTs65Dnmice(VoronovSVetal.,2008).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
Tissue-specificalternativesplicinggeneratestwosynaptojaninisoformswithdifferentialmembranebindingproperties(1996).RamjaunAR,McPhersonPS.JBiolChem.Oct4;271(40):24856-61.
Synaptojanin1-linkedphosphoinositidedyshomeostasisandcognitivedeficitsinmousemodelsofDown’ssyndrome(2008).VoronovSV,FrereSG,GiovediS,PollinaEA,BorelC,ZhangH,SchmidtC,AkesonEC,WenkMR,CimasoniL,ArancioO,DavissonMT,AntonarakisSE,GardinerK,DeCamilliP,DiPaoloG.ProcNatlAcadSciUSA.Jul8;105(27):9415-20.
