Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
- Applications:
- WB1:1,000IHC1:400
- Species:
- Rabbit
- MolecularReference:
- ~60kDa-62kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1570-309,RRID:AB_2492253
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser309ofsynaptotagmin.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Synaptotagminiswidelyregardedastheprimarycalciumsensorforsynapticvesicleexocytosis(Fernandez-Chaconetal.,2001;Wangetal.,2003).Moreover,recentstudiesindicatethattheproteinalsoplaysakeyroleinendocytosis(Poskanzeretal.,2003).Synaptotagmincanbephosphorylatedbymultipleproteinkinasesandthismayplayakeyroleinmodulationofsynaptotagmin’sABIlitytoinfluenceboththeexocytoticandendocytoticcomponentsofsynaptictransmission(Hilfikeretal.,1999;Leeetal.,2004).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
SergioLeal-Ortiz,ClarissaL.Waites,RyanTerry-Lorenzo,PedroZamorano,EckartD.Gundelfinger,andCraigC.Garner(2008)PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.J.CellBiol.,181:831–846.PMID:18519737
