Mousemonoclonalantibody
- Size:
- 100µl
- Formulation:
- ProteinGPurified
- Specificity:
- Rat,Human,Porcine
- Applications:
- WB1:1,000IHC1:100
- Species:
- Mouse
- MolecularReference:
- ~36kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#1990-STX,RRID:AB_2492254
Antigen/Purification:Collapse
Theantigenisasynapticimmunoprecipitate(crude)fromhumanbrain.
TheantibodyisProteinGpurifiedculturesupernatant.
BIOLOGicalSignificance:Collapse
Syntaxinsareafamilyofproteinsinvolvedinthedockingofsynapticvesicleswiththeplasmamembrane.Syntaxin,alongwithsynaptobrevinandSNAP-25,formstheSNAREcomplexwhichmediatessynapticvesiclefusionandexocytosis.BindingofMunc18-1totheN-terminusofsyntaxin1hasbeendemonstratedtobeessentialforexocyticmembranefusion(Khvotchevetal.,2007).Decreasedlevelsofphosphorylatedsyntaxin1intheprefrontalcortexhaverecentlybeencorrelatedwithschizophrenia(Castilloetal.,2010).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
KhvotchevM,DulubovaI,SunJ,DaiH,RizoJ,SüdhofTC(2007)DualmodesofMunc18-1/SNAREinteractionsarecoupledbyfunctionallycriticalbindingtosyntaxin-1Nterminus.JNeurosci.2007Nov7;27(45):12147-55
CastilloMA,GhoseS,TammingaCA,Ulery-ReynoldsPG(2010)Deficitsinsyntaxin1phosphorylationinschizophreniaprefrontalcortex.BiolPsychiatry.67(3):208-16
Honer,W.G.,Hu,L.&Davies,P.Humansynapticproteinswithaheterogeneousdistributionincerebellumandvisualcortex.BrainResearch609,9-20(1993).
