Rabbit polyclonal antibody

Antigen/Purification: ExpandCollapse

The antigen is a phosphopeptide corresponding to amino acid residues surrounding phospho-Ser⁸⁶² of rat mGluR7.

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide columns.

Biological Significance: ExpandCollapse

Metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). The mGluRs are divided into three groups based on sequence identity and pharmacological properties: group I (mGluR1 and mGluR5) are localized in the perisynaptic region of the postsynaptic membrane, whereas group II (mGlur2 and mGluR3) and group III (mGluR4,6,7 and 8) are localized predominantly at presynaptic terminals. PKC phosphorylation of serine⁸⁶² on mGluR7 has been shown to be critical for stabilizing receptor surface expression and promoting binding to the synaptic PDZ-domain-containing protein PICK1 (Suh et al., 2008).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

General References

Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci 24:8752-8761.

Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res

Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London) 410:588-592.

Suh YH, Pelkey KA, Lavezzari G, Roche PA, Huganir RL, McBain CJ, Roche KW. (2008) Corequirement of PICK1 binding and PKC phosphorylation for stable surface expression of the metabotropic glutamate receptor mGluR7. Neuron. 58(5):736-48