Rabbit polyclonal antibody

Antigen/Purification: ExpandCollapse

The antigen is a  phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr³⁴² of Drosophila AKT.

The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Biological Significance: ExpandCollapse

The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr³⁴², and the other in the C-terminal regulatory domain, Ser⁵⁰⁵: these two activation sites are homologous to mammalian Ser⁴⁷³ and Thr308 respectively (Powell et al., 2004).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Schleich S, Strassburger K, Janiesch PC, Koledachkina T, Miller KK, Haneke K, Cheng YS, Küchler K, Stoecklin G, Duncan KE, Teleman AA (2014) DENR-MCT-1 promotes translation reinitiation downstream of uORFs to control tissue growth. Nature. Aug 14;512(7513):208-12. doi: 10.1038/nature13401.  PMID: 25043021